DRAGEN NextSeq 2000 Pūnaha Whakaraupapa

Whakatakotoranga

• Ingoa Hua: DRAGEN Pūtau Kotahi RNA v4.2.11 mo NextSeq 1000/2000
• Nama Wāhanga: N/A
• Nama Tuhinga: 200074785
• Putanga: 00
• Te Ra Whai Mana: 19-Mahuru-2025 04:33 ki runga (KORE)

WHAKATOKANGA

Ko enei Release Notes e whakaatu ana i nga huringa matua ki te DRAGEN Single Cell RNA Workflow i runga i te taputapu NextSeq 1000/2000, mai i te putanga v4.2.7. Mena kei te whakahou koe mai i tetahi putanga tawhito, tirohia nga korero tuku takawaenga katoa.

NGA PANUITANGA I V4.2.11

• Te tātaritanga scRNA
  • Kua whakatikahia te take i he te whakahaere i te Raarangi Fastq Kawemai i te R1 kau
• I runga i te taputapu motuhake
  • Tāpiri tautoko mo te Oracle9 OS
  • Te whakapai ake i te pakari mo nga tātaritanga i runga i te papa, me te whakatika mo te kore e kati
  • I whakatikahia he hapa whakamana i mua i te whakahaere i te wa ka tohua te kopeke Ora engari kaua he FASTQ e pupuri
  • Kua whakatikahia he pepeke hurihanga pororakau i whakaemi ai nga poro ki runga i te taputapu
  • Tango i te R me te Rscript whakawhirinaki mai i te kaitautauta, hei whakatau i te rapunga haumarutanga ipurangi.

HE KORERO

Tohutoro Tohutoro Whakamahinga Tohutoro
Germline, Whakarangatira Germline, Amplicon Germline Rerengamahi

• Whakamahia te mapi-a-ALT.
• Whakamahia nga tohu ira kauwhata.
• Whakamahia te Homo sapiens [1000 Genomes] hg38 Alt Masked Graph v3 tohutoro tohutoro.
• Ki te kore e whakamahi hg38, ka tūtohutia te Homo sapiens [UCSC] hg19 Alt Masked Graph v3, Homo sapiens [NCBI] hs37d5 v3 Graph.
• Ka tautokohia te kauwhata-kore, engari kua iti ake te tika

Whakarangatira Somatic, Amplicon Somatic, RNA me scRNA Rerengamahi 

• Whakamahia te mapi-a-ALT.
• Whakamahia nga ira-kauwhata-kore.
• Whakamahia te Homo sapiens [1000 Genomes] hg38 Alt Masked v3 reference genome or Homo sapiens [UCSC] hg19 Alt Masked v3 reference genome.

Ripanga 1 v4.2 Tautoko Tohutoro me te Whakamahinga Manaakitanga mo nga Raraunga Tangata

Tangata		hg19	hs37d5	hg38	chm13	Momo Tohutoro Tohutoro
Germline	SNV	Ae	Ae	Ae	Ae	Kauwhata
	CNV	Ae	Ae	Ae	Ae*	Kauwhata
	SV	Ae	Ae	Ae	Ae*	Kauwhata
	Whakawhanui Hunter	Ae	Ae	Ae	Kao	Kauwhata

	Nga Kai-waea kua whakaritea	Ae	Ae	Ae	Kao	Kauwhata
	RNA	Ae	Ae	Ae	Ae*	Kauwhata-kore
	De Novo	Ae	Ae	Ae	Ae*	Kauwhata
	Te Tohua-a-Kiwa	Ae	Ae	Ae	Ae*	Kauwhata
	Kaitohu koiora (HLA)	Ae	Ae	Ae	Ae*	Kauwhata
	Gvcf Genotyper	Ae	Ae	Ae	Ae*	Kauwhata
Somatic	SNV	Ae	Ae	Ae	Ae*	Kauwhata-kore
	UMI SNV	Ae	Ae	Ae	Ae*	Kauwhata-kore
	CNV	Ae	Ae	Ae	Ae*	Kauwhata-kore
	SV	Ae	Ae	Ae	Ae*	Kauwhata-kore
Methylation	Methylation	Ae	Ae	Ae	Kao	Kauwhata-kore
Panuitanga	Nirvana	Ae	Ae	Ae	Kao	n/a

(*) Ka tautoko a DRAGEN™ i te mahinga o nga waahanga; heoi, kare ano kia tau te tika o te waahanga.

Ripanga 2 v4.2 Tautoko Tohutoro me te Whakamahinga Manaakitanga mo nga Raraunga Kore-tangata Ehara i te Tangata

Kore-Tangata		Tautokohia	Momo Tohutoro Tohutoro
Germline	SNV	Ae	Kauwhata-kore
	CNV	Kao	n/a
	SV	Ae	Kauwhata-kore
	Whakawhanui Hunter	Kao	n/a
	Nga Kai-waea kua whakaritea	Kao	n/a
	RNA	Ae	Kauwhata-kore
	De Novo	Ae	Kauwhata-kore
	Te Tohua-a-Kiwa	Ae	Kauwhata-kore
	Kaitohu koiora (HLA)	Kao	n/a
	Gvcf Genotyper	Ae	Kauwhata-kore
Somatic	SNV	Kao	n/a
	UMI SNV	Kao	n/a
	CNV	Kao	n/a
	SV	Kao	n/a
Methylation	Methylation	Kao	n/a
Panuitanga	Nirvana	Ae	n/a

Me pehea te Whakahou i nga Ira Tohutoro Taputapu 

Ka taea e koe anake te kawemai i nga ira tohutoro hou mai i te kaute kaiwhakahaere.

Hipanga: 

1. Tikiake i te mōkihi genome tar.gz mai i te whārangi NextSeq 1000/2000 Software Downloads, RĀNEI
2. Waihangahia he momo tohutoro ma te whakamahi i te Kaihanga Tohutoro mo nga taputapu Illumina BaseSpace Sequence Hub taupānga. Mo etahi atu korero, tirohia te Kaihanga Tohutoro mo Illumina Instruments v1.0.0 App Awhina Online.
3. Tīpakohia te tahua pūmanawa mana, ka tīpako Whakahaere Tukanga. Me whakarite karekau he oma raupapa, he tātaritanga tuarua i runga taputapu kei te haere tonu.
4. Mai te tahua pūmanawa mana, tīpako Whakaitihia te Taupānga.
5. Takiuru ki ilmnadmin.
6. Tīpakohia te tahua pūmanawa whakahaere, ka tohua DRAGEN.
7. I roto i te Genome wāhanga, tīpako View Kua whakauruhia nga Genomes ki view he rarangi o nga momo ira katoa kua whakauruhia inaianei.
8. Katia te matapihi.
9. I raro i te Kawemai i nga Genomes Tohutoro Hou, tohua Whiriwhiria.

Whakatere ki te ira tohutoro file (*.tar.gz) i runga i te puku whatunga kawe, kua whakairihia ranei, ka kowhiria te Tuwhera.

NGA PUTANGA TINO

Kua whakatikahia nga take o te DRAGEN™ v4.2.11 Reremahi RNA Pūtau Kotahi

Waehanga	ID hapa	Putanga Whakaahuatanga
scRNA	DRAGEN- 20879	Whakatikatika mo te scRNA.barcodeSummary.csv pūtau kotahi e whakaatu ana i nga kaute UMI hē
scRNA	TAKENA– 24741	Whakatikahia kia hainatia i mua URLs mo UMI filekei roto i te rarangi FASTQ.csv
scATAC	DRAGEN- 22854, SET- 4565	Whakatikatika mo te Ahuatanga/Tauturu ID kua ngaro i te putanga scRNA/scATAC
QC Metrics, scRNA	DRAGEN- 15950	Whakatikahia he rereke rere-ki-rere i te scRNA i runga i te bdbio
scRNA	DRAGEN- 16360	Whakatikatika mo te DRAGEN i NextSeq 2000: Whakairihia te RNA Pūtau Takitahi
scRNA	DRAGEN- 15329	Whakatikahia te maha o nga urunga kore-kore i te matrix.mtx file (te tatau āhuatanga)

NGA TOHUTOHU WHAKATANGA

Ko nga rerengamahi DRAGEN v4.2.11 he hototahi ki te Pūmanawa Mana 2.0.x. Mo nga korero e pa ana ki te hototahi ki etahi atu putanga Pūmanawa Mana, tirohia te whaarangi NextSeq 1000/2000 Hua Hototahi i te pae tautoko Illumina. Mena kei te pirangi koe ki te whakahou i te punaha whakahaere, me whai i nga hikoinga kua whakamaarama i te NextSeq 1000/2000 Control Software Suite v2.0.x Release Notes on the Illumina Support Site.

Tāutatanga Ipurangi o Rerengamahi
Mena ka hono te taputapu ki te ipurangi, ka taea e koe te whakauru tika i nga rerengamahi DRAGEN mai i te Pūmanawa Mana. Kei te waatea te whakaurunga o nga rerengamahi mai i te Pūmanawa Mana v1.3 i muri mai ranei.

Hipanga:

1. Kia mohio kei a koe te kupuhipa ki te kaute ilmnadmin.
2. Takiuru ki te pūkete ilmnadmin:
   • a. Mena kua takiuru koe hei ilmnuser me te punaha whakahaere, tohua te tahua raupaparorohiko whakahaere, ka kowhiri i te Putanga Tono kia uru atu ki te papamahi.
   • b. Tīpakohia te tohu pātene mana i te kokonga matau o runga ka takiputa atu i te ilmnuser.
   • c. I muri i a koe i runga i te mata takiuru, tohua te ilmnadmin, ka uru ki te kupuhipa hei takiuru.
   • d. Ka whakarewa aunoa te punaha whakahaere ina kua takiuru koe.
3. Me whakarite karekau he oma raupapa, he tātaritanga tuarua i runga taputapu kei te ahu whakamua.
4. I runga i te tahua pūmanawa mana, tīpako DRAGEN. I raro i te Putanga, ka whakarārangihia e te wāhanga Reremahi Wātea ngā rerengamahi kua tāutatia i tēnei wā ki te pūnaha.
5. Tīpakohia Tirohia Online. Ko nga putanga DRAGEN me nga rerengamahi katoa kaore i te hototahi ki te whakaurunga ipurangi. Whakamahia te whakaurunga tuimotu mo etahi atu rerengamahi.
6. Tīpakohia te pouakataki mo nga rerengamahi e hiahia ana koe ki te whakauru. FAKATOKANGA: Mo nga Whakahoutanga Ipurangi, me whakauru te DRAGEN BCL Convert i mua, i etahi atu rerengamahi o te Putanga DRAGEN kotahi. Ka taea e koe view nga korero mo te putanga hou o te rerengamahi i roto i nga korero tuku.
7. Tīpakohia te Tāuta hei tīmata i te tāutanga.
8. Whakauruhia te ilmnadmin mo te kupuhipa punaha, ka kowhiria te Motuhēhē.
9. Ka oti te whakaurunga, ka hoki koe ki te mata DRAGEN ka taea view te rarangi whakahou o nga rerengamahi DRAGEN kua whakauruhia.

Tāuta Tuimotu o Rerengamahi 

Hipanga: 

1. Ina watea mai he whakahou rerengamahi DRAGEN, tikina te kaiwhakaputa (*.tar.gz) mai i te wharangi tautoko NextSeq 1000/2000 Sequencing System. Tiakina te kaiwhakaputa ki te puku o te takiwa, te puku kawe ranei. Tuhipoka: i te iti rawa, me tango me te whakauru i te whakahou DRAGEN BCL Convert o te Putanga DRAGEN kotahi.
2. Mēnā i tiakina e koe te pūtāuta ki te puku kawe, monohia te puku ki te tauranga USB 3.0, kei te taha e rua me te tuara o te taputapu. Whakanuia te taputapu ina hiahiatia kia uru atu ki muri.
3. A pee i nga taahiraa 1-3 i runga ake nei ki te takiuru ki ilmnadmin.
4. Tīpakohia te tahua pūmanawa whakahaere, ka tohua DRAGEN.
5. I raro i te Putanga, tohua Tirotiro mo te Putanga Hou ki te whakatere ki te kaiwhakaputa. Tuhipoka: Me whakauru te DRAGEN BCL Tahuri o te Putanga DRAGEN kotahi i mua i etahi atu rerengamahi.
6. Tīpakohia te Tāuta hei tīmata i te tāutanga.
7. Whakauruhia te ilmnadmin mo te kupuhipa punaha, ka kowhiria te Motuhēhē.
8. Ka oti te whakaurunga, ka hoki koe ki te mata DRAGEN ka taea view te rarangi whakahou o nga rerengamahi DRAGEN kua whakauruhia.

Kia mahara ka timata me te mana whakahaere v1.5, ka taea te tango i nga putanga o mua o nga rerengamahi DRAGEN.

WHAKATOKANGA TE KORERO

Arotakenga	Tuku Tohutoro	Taketake	Whakaahuatanga o Huringa
00	CN 1127648	Heta Gwin	Tukunga tuatahi

Whakapā

• Taitara: DRAGEN Pūtau Kotahi RNA v4.2.11 mo NextSeq 1000/2000 Panui Tukunga Kaihoko
• Nama Wāhanga: N/A
• Tau Tuhinga: 200074785, Putanga: 00
• Te Ra Whai Mana: 19-Mahuru-2025 04:33 ki runga (KORE)

FAQ

P: Kia pehea te maha o taku whakahou i nga tohu tohutoro i runga i te taputapu?

A: E taunaki ana ki te whakahou i nga tohu tohutoro i nga wa katoa ka tukuna he putanga hou hei whakarite i te hototahi me te mahi tino pai.

Q: Me aha ahau ki te tupono ahau ki te hapa whakamana i mua i te whakahaere?

A: Mena kei te raru koe i te hapa whakamana i mua i te whakahaerenga, me whakarite kia tika te tohu o te kopeke Ora ka tirohia mena kei te waatea nga FASTQ e hiahiatia ana. Mena kei te haere tonu te raru, tirohia te waahanga rapurongoā o te pukapuka.

Tuhinga / Rauemi

DRAGEN NextSeq 2000 Pūnaha Whakaraupapa [pdf] Pukapuka Tohutohu NextSeq 1000, NextSeq 2000, NextSeq 2000 Pūnaha Whakaraupapa, Pūnaha Whakaraupapa, Pūnaha

Tohutoro

• Pukapuka Kaiwhakamahi