Dynamic Biosensors helix cyto Normalization Solution
Whakatakotoranga
- Ingoa Hua: heliX cyto Normalization Solution (Red Dye)
- Tau ota: NOR-R2
- For scIC measurements in the red channel
- Mo te whakamahi rangahau anake
- Limited shelf life – please check expiry date on label
Nga waahanga matua
- Mo te whakatikatika i nga tohu korikori i runga i te Wahi 1 me te Wahi 2 o te maramara heliXcyto
- Enables correct real-time referencing of the red fluorescent signals during scIC measurements
- Compatible with all heliXcyto chips
- The Normalization solution (red dye) contains a hydrophilic red dye with a single negative net charge.
Whakaahuatanga Hua
- Tau ota: NOR-R2
Ripanga 1. Nga Ihirangi me nga Moohiohio Rokiroki
| Rauemi | Cap | Concentr ation | Te nui | Rokiroki |
| Normalization solution-R2 | Whero | 10 µM | 6x 100 µL | -20°C |
- Mo te whakamahi rangahau anake.
- This product has a limited shelf life; please see the expiry date on the label.
- To avoid many freeze-thaw cycles, please aliquot the solution.
Whakaritenga
- Use this red dye normalization solution for scIC measurements in the red channel (analyte label-dependent).
- Dilute the 10 µM normalization stock solution to a working concentration with running buffer.
- The concentration of the normalization solution should approximately correspond to the fluorophore concentration in the highest analyte concentration to be measured. This can be calculated usingthe following equation:
Concentration of the normalization solution in the desired color :
Te kukū o te waikano ki te wairewa tātari kua tapaina
Highest concentration of the analyte that should be measured
Degree of labeling (ratio of dye to analyte)
Ka taea te rongoa i nga otinga kua waimeha ki te 2-8°C mo te 7 ra.
Panui Tono
In the scIC measurement, the fluorescent signal of the normalization solution should be in a similar range to the highest signal coming from the bound analyte (raw data). The absolute fluorescent signal is dependent on normalization solution concentration and the excitation power applied in the measurement. The excitation power has to be selected based on the following parameters:
- Fluorophore concentration inthe analyte solution
Ko te kukū o te fluorophore kei runga i te kukū wetewete e whakamahia ana i roto i te ine me te tohu tohu o te tātari. Mo te DOL teitei me te nui o te kuhanga tātari, me whakaheke te mana whakakorikori. - Te tohu here e tumanakohia ana
Highly expressed targets on a cell can bind more molecules of the labeled analyte. In case of highly overexpressed targets, a strong binding signal can be expected. To avoid the shutter closing, lowering the excitation power might be considered. - Momo Chip
He rereke nga ahuatanga o nga momo maramara rereke. Ko te nui ake o nga mahanga me te nui ake o nga mahanga i runga i te maramara, ka teitei ake te tohu papamuri. No reira, he iti ake te mana whakakorikori o nga maramara L5 i te tono ki nga maramara M5.
For a starting point of excitation power and normSolution on concentration to be used in an scIC experiment, please refer to Table 2.
Table 2. Relation of fluorophore concentration, normalization solution concentration, and excitation power suitable for an 
M5 maramara
| Waikano tātari conc. = tātari conc x DOL | Te mana whakahihiri | Rongonga Whakaotahitanga Kukū | Wairewa Whakatauritenga |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 µM | 0.05 | 1 µM | 1:10 |
| 2.5 µM | 0.02 | 2.5 µM | 1:4 |
Tuhipoka: This table is for your guidance. However, the final signal recorded in the heliXcyto depends on many factors. Thus, some optimization will be required for each system.
Whakapā
Dynamic Biosensors GmbH
Perchtinger Str. 8/10 81379 Munich, Germany
Bruker Scientific LLC
40 Manning Road, Manning Park, Billerica, MA 01821 USA
- Nga korero ota
- Tautoko Hangarau
www.dynamic-biosensors.com
Ko nga taputapu me nga maramara ka hangaia, ka hangaia i Germany. ©2025 Dynamic Biosensors GmbH Mo te Whakamahi Rangahau Anake. Kaua e whakamahia i roto i nga tikanga tātaritanga haumanu.
FAQ
What is the shelf life of the heliX cyto Normalization Solution?
He iti te oranga o te hua. Tena tirohia te ra paunga i runga i te tapanga mo nga korero motuhake.
How should I store the NOR-R2 v1.0 product?
The product should be stored according to the storage information provided in Table 1 of the user manual.
Can I use this product for clinical purposes?
No, this product is for research use only.
Tuhinga / Rauemi
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Dynamic Biosensors helix cyto Normalization Solution [pdf] Pukapuka Kaiwhakamahi helix cyto Normalization Solution, helix cyto, Normalization Solution, Solution |