BIOSENSORS hihiri heliXcyto Rongoa Whakapapa
Nga waahanga matua
- Mo te whakatikatika i nga tohu rewharewha ki te Wahi 1 me te Wahi 2 o te maramara cyto heliX
- Whakahohehia te tohutoro tika mo nga tohu tohu whero whero, kaakariki ranei i nga inenga RT-IC
- Hototahi ki nga maramara heliX cyto katoa
- Ko te otinga Normalization-Ra he waikano whero ngawari me te utu kupenga pai kotahi
- Ko te otinga Normalization-Ga he waikano kaakaariki hydrophilic me te utu kupenga kino kotahi
Whakaahuatanga Hua
Tau ota: KORE-0
Ripanga 1. Ihirangi me nga korero mo te rokiroki
| Rauemi | Cap | Te aro | Te nui | Rokiroki |
| Whakataurite otinga-Ra | Karaka | 10 µM | 3x 100 µL | -20 °C |
| Whakataurite otinga-Ga | Kakariki | 10 µM | 3x 100 µL | -20 °C |
Mo te whakamahi rangahau anake.
He iti te ora o tenei hua, tirohia koa te ra paunga i runga i te tapanga.
Whakaritenga
Kōwhiria te tae otinga whakaōrite (Ga, Ra rānei) e hāngai ana ki tō tae tapanga tātari. Waimehaehae i te wairewa kararehe whakarite 10 µM ki te kukū e mahi ana me te parare rere.
Ko te pukapuka whakamutunga e hiahiatia ana mo te whakamatautau ka kitea i roto i nga heliOSampte paepae view o te whakamatautau.
Ko te kukū o te otinga whakarite kia rite ki te kukū fluorophore i roto i te kukū tātaritanga teitei rawa hei ine. Ka taea te tatau tenei ma te whakamahi i te wharite e whai ake nei:
: Te kukū o te otinga whakaōrite ki te tae e hiahiatia ana
: Te kukū o te waikano ki te wairewa tātari kua tapaina
: Te kukū teitei o te wetewete me ine
: Te tohu tohu (te owehenga o te waikano ki te tātari)
Ka taea te rongoa i nga otinga kua waimeha ki te 2-8°C mo te 7 ra.
NUI: Kaua e whakahuihuitia te otinga Whakapaipai-Ra me te otinga Whakapaipai-Ga, mena kei te whakarite koe i te inenga Tae-rua (te panui whakarara o te hongere matomato me te whero).
Panui Tono
I roto i te inenga RT-IC, me noho te tohu rewharewha o te otinga whakanoa ki a awhe rite hei tohu teitei rawa mai i te wetewete herea (raraunga mata).
Ko te tohu rewharewha tino e ti'aturi ana ki te kukū o te otinga whakarite me te mana whakakorikori e whakamahia ana i roto i te inenga. Me kowhiria te mana whakaoho i runga i te tawhā e whai ake nei:
a. Te kukū o te Fluorophore i roto i te wairewa tātari:
Ko te kukū o te fluorophore kei runga i te kukū wetewete e whakamahia ana i roto i te ine me te tohu tohu o te tātari. Mo te DOL teitei me te nui o te kuhanga tātari, me whakaheke te mana whakakorikori.
b. Tohu herenga e tumanakohia ana:
Ka taea e nga whainga e tino kitea ana i runga i te pūtau te here i etahi atu ngota ngota o te wetewete kua tapaina. I te wa o nga whaainga kua tino whakahuahia he tohu here kaha ka taea te tumanako. Hei karo i te katinga o te marumaru, ka whakaarotia pea te whakaheke i te mana whakaoho.
c. Momo maramara:
He rereke te papamuri o nga momo maramara rereke. Ko te nui ake o nga mahanga me te nui ake o nga mahanga i runga i te maramara, ka teitei ake te tohu papamuri. No reira, he iti ake te mana whakakorikori o nga maramara L5 i te tono ki nga maramara M5.
Mo te timatanga o te mana whakahihiri me te tikanga. te kukū o te otinga hei whakamahi i roto i te whakamatautau RT-IC, tirohia te Ripanga 2.
Ripanga 2. Te hononga o te kukū fluorophore, te kukū otinga whakaōrite, me te mana whakakorikori e tika ana mo te maramara heliX cyto M5
| Waikano tātari conc. = tātari conc x DOL | Te mana whakahihiri | Rongonga Whakaotahitanga Kukū | Wairewa Whakatauritenga |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 µM | 0.05 | 1 µM | 1:10 |
| 2.5 µM | 0.02 | 2.5 µM | 1:4 |
Tuhipoka: Ko tenei tepu hei arahi i a koe. Engari, ko te tohu whakamutunga ka tuhia ki te heliX cyto ka whakawhirinaki ki nga mea maha. No reira, ka hiahiatia etahi arotautanga mo ia punaha.
Whakapā
| Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. |
| Perchtinger Str. 8/10 | 300 Trade Center, Suite 1400 |
| 81379 Munich | Woburn, MA 01801 |
| Tiamana | USA |
Nga korero ota order@dynamic-biosensors.com
Tautoko Hangarau support@dynamic-biosensors.com
Tautoko Kiritaki
www.dynamic-biosensors.com
Ko nga taputapu me nga maramara ka hangaia, ka hangaia i Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Pūmau te mana.
Tuhinga / Rauemi
 |
BIOSENSORS hihiri heliXcyto Rongoa Whakapapa [pdf] Pukapuka Kaiwhakamahi NOR-0, heliXcyto Normalization Solution, heliXcyto, Normalization Solution, Solution |
